Parainfluenza Virus Type 3, Hemagluttinin (FITC)
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Catalog number
MBS602530
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Price
Please ask
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Size
1 mL
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Products_type
Antibody
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Products_short_name
[Parainfluenza Virus Type 3, Hemagluttinin]
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Products_name_syn
[Anti -Parainfluenza Virus Type 3, Hemagluttinin (FITC)]
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Clonality
Monoclonal
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Clone
[5E404]
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Specificity
Specific for hemagluttinin of Parainfluenza virus, type 3.
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Purity
Affinity Purified > 90% pure mouse monoclonal antibody which has been purified from ascites fluid or culture
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Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added. Conjugated to FITC.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Immunofluorescence (IF)
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Short name
Parainfluenza Virus 3, Hemagluttinin (FITC)
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Technique
FITC, Fluorescein
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Host
Mouse
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Isotype
IgG2a
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Label
FITC
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Species
Virus, Viruses
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Alternative name
Parainfluenza Virus classification 3, Hemagluttinin (fluorecein)
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Alternative technique
fluorescine
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Virus
parainfluenza
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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