Mouse IgG3-FITC
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Catalog numberMBS666485
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PricePlease ask
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Size0.1 mg
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Products_typeAntibody
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Products_short_name[IgG3]
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Products_name_syn[Monoclonal Mouse IgG3/FITC]
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Products_gene_name[IgG3]
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ClonalityMonoclonal
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Clone[SIPC3385]
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Form50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
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Concentration0.5 mg/ml
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Storage_stabilityStore at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light Product should retain activity for at least 12 months after shipping date when stored as recommended.
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Tested_applicationFlow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA)
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse IgG3-FITC
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TechniqueMouse, FITC, Fluorescein, mouses
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HostMouse
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IsotypeIgG3k, IgG3
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameMouse IgG3-fluorecein
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Alternative techniquemurine, fluorescine
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data