Mouse Anti-Porcine CD21-FITC

• Catalog number
  MBS673826
• Price
  Please ask
• Size
  5x0.5 mg

• Products_type
  Antibody
• Products_short_name
  [CD21]
• Products_name_syn
  [Mouse Anti-Porcine CD21 - FITC; Mouse Anti-Pig CD21]
• Clonality
  Monoclonal
• Clone
  [BB6-11C9.6]
• Reactivity
  Porcine
• Specificity
  Porcine CD21, Mr 160 kDa CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells.1 Low density CD21 has also been reported on granulocytes and monocytes. MAb BB6-11C9.6 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97-100% in ileal Peyer's patches (ILPP); 93-97% in spleen; and 0% in thymus.2 The antibody does not stain B cells in cord blood or spleen from pig fetuses (105 days gestation).2
• Form
  Fluorescein (FITC) Conjugate
• Storage_stability
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
• Tested_application
  Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD21  
• Gene symbol
  CR2
• Short name
  Mouse Anti-Porcine CD21-FITC
• Technique
  anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
• Host
  Mouse
• Isotype
  Mouse (BALB/c) IgG1 kappa
• Label
  FITC
• Species
  Porcine, Mouses
• Alternative name
  Mouse Antibody topig CD21-fluorecein
• Alternative technique
  murine, antibodies, fluorescine

Gene info

• Identity
  HGNC:2336
• Gene
  CR2
• Long gene name
  complement C3d receptor 2
• Synonyms gene name
  • complement component (3d/Epstein Barr virus) receptor 2
  • complement component 3d receptor 2
• Synonyms
  • CD21
  • C3DR
• Synonyms name
  • Epstein-Barr virus receptor
• GenBank acession
  • M26004
• Locus
  1q32.2
• Discovery year
  2001-06-22
• Entrez gene record
  1380
• RefSeq identity
  • NM_001877
• Classification
  • Sushi domain containing
  • Complement system regulators and receptors
  • CD molecules
• VEGA ID
  OTTHUMG00000036307
• Locus Specific Databases
  • LRG_348

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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