Mouse Anti-Mouse I-Ek-BIOT
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Catalog numberMBS673650
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Price:
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Size0.5 mg
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Products_typeSecondary Antibody
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Products_short_name[Mouse Anti-Mouse I-Ek]
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Products_name_syn[Mouse Anti-Mouse I-Ek - Biotin; Mouse Anti-Mouse I-Ek]
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ClonalityMonoclonal
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Clone[14-4-4S]
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ReactivityMouse
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SpecificityAn epitope on mouse I-Ek MHC class II alloantigen The monoclonal antibody 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes.1 Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2 The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D.3
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FormBiotin (BIOT) Conjugate
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Storage_stabilityThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested_applicationFlow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse Anti-Mouse I-Ek-BIOT
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Techniqueanti-, Mouse, anti, antibody to, antibodies, mouses
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HostMouse
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IsotypeMouse (C3H.SW) IgG2a kappa
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse I-Ek-BIOT
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Alternative techniquemurine, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data