Mouse anti Mitotic cells
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Catalog numberMBS570109
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PricePlease ask
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Size0.1 mg
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Products_typeAntibody
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Products_short_name[Mitotic cells]
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ClonalityMonoclonal
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Clone[8B3G]
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ReactivityHuman, Zebrafish
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Specificity8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorpoRation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle.
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FormEach vial contains 100 ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
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Storage_stabilityStore at 4 degree C, or in small aliquots at -20 degree C.
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Tested_applicationFlow Cytometry (FC), Immunocytochemistry (IHC)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse anti Mitotic cells
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TechniqueMouse, anti, antibody to, mouses
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HostMouse
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IsotypeIgM
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to Mitotic cells
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Alternative techniquemurine, antibodies
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Tissuecells
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data