Mouse anti Human IgE (class specific), conjugated with FITC

  • Catalog number
    MBS571075
  • Price
    Please ask
  • Size
    5x0.2 mg
  • Products_type
    Secondary Antibody
  • Products_short_name
    [Mouse anti Human IgE (class specific)]
  • Clonality
    Monoclonal
  • Clone
    [NI 307]
  • Reactivity
    Human
  • Specificity
    Fluorescein isothiocyanate-conjugated purified monoclonal Mouse antibody to Human IgE, isotype specific.
  • Storage_stability
    Store at 4 degree C.
  • Tested_application
    ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Mouse anti IgE (class specific), conjugated with FITC
  • Technique
    anti human, Mouse, anti, FITC, IgE, antibody to, anti human antibodies, antibody Conjugates, Fluorescein, IgEs, mouses
  • Host
    Mouse
  • Isotype
    IgG1, IgE
  • Label
    FITC
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse antibody to H. sapiens immunoglobuline E (class specific), coupled including fluorecein
  • Alternative technique
    murine, antibodies, fluorescine, immunoglobulin-e
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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