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Products_type
Antibody
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Products_short_name
[CD3]
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Products_name_syn
[Mouse Anti-Human CD3 - Allophycocyanin; Mouse Anti-Human CD3]
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Other_names
[CD3g molecule, gamma (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3gamma/delta; CD3 molecule, gamma/delta (CD3-TCR complex); T-cell surface glycoprotein CD3 gamma/delta chain homolog; CD3g molecule, gamma (CD3-TCR complex)
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Other_gene_names
[cd3g; CD3; t3g; cd3gd; cd3g-a; cd3g/d; cd3-gamma]
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Clonality
Monoclonal
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Clone
[UCHT1]
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Reactivity
Human
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Specificity
epsilon chain of the CD3/T cell antigen receptor complex CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa epsilon chain of the CD3/TCR complex.1-5
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Form
Allophycocyanin (APC) Conjugate
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Storage_stability
The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC), Cyanine 5 (CY 5), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), and allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested_application
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
MyBioSource supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Latin name
Mus musculus