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Products_type
Secondary Antibody
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Products_short_name
[Mouse anti Human Bence Jones lambda (surface and hidden determinants)]
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Clonality
Monoclonal
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Clone
[NI 412, NI 268]
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Reactivity
The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
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Specificity
The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining.
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Form
Delipidated, heat inactivated, lyophilized, stable whole ascites.
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Storage_stability
Store at 4 degree C. Handling: The lyophilized product is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 degree C, not refrozen, and preferably used the same day. Lyophilized at +4 degree C = at least 10 years reconstituted at or below -20 degree C = 3-5 years reconstituted at +4 degree C = 7 days
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Tested_application
Indirect Immunofluorescence (IF), ELISA (EIA)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus