Mouse Anti-Chicken CD8b-FITC
-
Catalog number
MBS673903
-
Price
Please ask
-
Size
0.5 mg
-
-
Products_type
Antibody
-
Products_short_name
[CD8 beta]
-
Products_name_syn
[Mouse Anti-Chicken CD8b - FITC; Mouse Anti-Chicken CD8 beta]
-
Other_names
[CD8 beta; CD8 beta]
-
Other_gene_names
[LOC100136222]
-
Clonality
Monoclonal
-
Clone
[EP42]
-
Reactivity
Chicken
-
Specificity
beta chain (Mr 34 kDa) of chicken CD8 In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha - and CD8 beta -chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but
-
Form
Fluorescein Conjugate
-
Storage_stability
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
-
Tested_application
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene target
-
Gene symbol
CD8B, CD8B2
-
Short name
Mouse Anti- CD8b-FITC
-
Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
-
Host
Mouse
-
Isotype
Mouse IgG2a kappa
-
Label
FITC
-
Species
chicken, Chickens, Mouses
-
Alternative name
Mouse Antibody toChicken CD8b molecule-fluorecein
-
Alternative technique
murine, antibodies, fluorescine
-
Alternative to gene target
CD8b molecule, CD8B and IDBG-60041 and ENSG00000172116 and 100996919,926, MHC class I protein binding, Extracellular, Cd8b1 and IDBG-155547 and ENSMUSG00000053044 and 12526, CD8B and IDBG-634906 and ENSBTAG00000008956 and 508633
-
Gene info
-
Identity
-
Gene
-
Long gene name
CD8b molecule
-
Synonyms gene
-
Synonyms gene name
- CD8 antigen, beta polypeptide 1 (p37)
-
Locus
-
Discovery year
1989-04-13
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- CD molecules
- V-set domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
CD8b2 molecule
-
Synonyms gene
-
Synonyms gene name
- CD8 antigen, beta polypeptide 2, pseudogene (p37)
- CD8b molecule pseudogene
-
Locus
-
Discovery year
1992-07-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Product images
Similar products
