Mouse Anti-Chicken CD45-FITC
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Catalog number
MBS673897
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Price
Please ask
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Size
5x0.5 mg
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Products_type
Antibody
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Products_short_name
[CD45]
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Products_name_syn
[Mouse Anti-Chicken CD45 - FITC; Mouse Anti-Chicken CD45]
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Other_names
[CD45; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; OTTHUMP00000033813; OTTHUMP00000033816; OTTHUMP00000033817; OTTHUMP00000038574; leukocyte common antigen; leukocyte-common antigen; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200]
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Other_gene_names
[PTPRC; PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; CD45]
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Clonality
Monoclonal
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Clone
[LT40]
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Reactivity
Chicken
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Specificity
Chicken CD45 (Mr 190 to 215-kDa). Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45.1 Levels of CD45 expression detected by MAb LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45lo) of thymocytes. The CD45lo population contains exclusively thymocytes with the CD3-CD4-CD8lo phenotype characteristic of the immediate precursors to the CD3- CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8lo to CD4+CD8+ and before the expression of CD3. The ability of MAb LT40 to discriminate this CD45lo subpopulation provides a suitable marker for the CD3-CD4-CD8lo immediate precursors to the CD3-CD4+CD8+ thymocytes.1
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Form
Fluorescein (FITC) Conjugate
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Storage_stability
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested_application
Flow Cytometry; Immunoprecipitation
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
PTPRCAP, PTPRC
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Short name
Mouse Anti- CD45-FITC
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Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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Host
Mouse
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Isotype
Mouse IgM kappa
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Label
FITC
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Species
chicken, Chickens, Mouses
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Alternative name
Mouse Antibody toChicken CD45-fluorecein
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Alternative technique
murine, antibodies, fluorescine
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
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Tree numbers
- E01.370.225.500.195.107.595.500.150
- E01.370.225.625.107.595.500.150
- E05.200.500.195.107.595.500.150
- E05.200.625.107.595.500.150
- E05.242.195.107.595.500.150
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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