MHC Class 2, RT1B (MHC Class II) (FITC)

• Catalog number
  MBS632694
• Price
  Please ask
• Size
  0.05 mg

• Products_type
  Antibody
• Products_short_name
  [MHC Class 2, RT1B]
• Products_name_syn
  [Anti -MHC Class 2, RT1B (MHC Class II) (FITC)]
• Clonality
  Monoclonal
• Clone
  [10F149]
• Reactivity
  Mouse, Rat
• Specificity
  Recognizes rat MHC class II RT1B. Species Crossreactivity: mouse.
• Purity
  Affinity Purified Purified by Protein G affinity chromatography.
• Form
  Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
• Storage_stability
  May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
• Tested_application
  Flow Cytometry (FC/FACS)
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  MHC   Class   RT1B   MHC   Class  
• Short name
  MHC Class 2, RT1B (MHC Class II) (FITC)
• Technique
  FITC, Fluorescein
• Host
  Mouse
• Isotype
  IgG1
• Label
  FITC
• Alternative name
  Major histocompabitility complex Class 2, RT1B (Major histocompabitility complex Class II) (fluorecein)
• Alternative technique
  fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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