MHC Class 1, H2, Db, alpha3 (Biotin)

Products_type

Antibody
Products_short_name

[MHC Class 1, H2, Db, alpha3]
Products_name_syn

[Anti -MHC Class 1, H2, Db, alpha3 (Biotin)]
Clonality

Monoclonal
Clone

[28-14-8]
Reactivity

Mouse
Specificity

Recognizes an epitope in the alpha3 domain of mouse H-2Db. Crossreacts with the alpha3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. Antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
Purity

Purified Purified
Form

Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin.
Storage_stability

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Tested_application

Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Gene target

MHC Class alpha3
Short name

MHC Class 1, H2, Db, alpha3 ( )
Host

Mouse
Isotype

IgG2a,k
Label

Biotin
Alternative name

Major histocompabitility complex Class 1, H2, Db, alpha3 (biotinilated)