MAb to FITC
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Catalog numberMBS311291
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Price:
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Size5x1 mg
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Products_typeAntibody
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Products_short_name[Fluorescein Isothiocyanate (FITC)]
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Products_name_syn[Monoclonal Antibody to Fluorescein Isothiocyanate (FITC)]
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ClonalityMonoclonal
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Clone[901]
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SpecificityFluorescein Isothiocyanate (FITC) Preferential binding of isomer 6, reduced binding to isomer 5
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PurityDEAE column chromatography
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FormPurified, Liquid
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Concentration4.36 mg/ml (OD280nm, E^1% = 14)
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Storage_stabilityShort-term (up to 6 months) store at 2 to 8 degree C. Long term, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Gene symbolMAB21L2, MAB21L1, MAB21L3
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Short nameMAb FITC
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TechniqueFITC, Fluorescein
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HostHost: Mouse. Hybridization of P3X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice. Source: Ascites
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IsotypeIgG1
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LabelFITC
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Alternative namemonoclonal to fluorecein
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Alternative techniquefluorescine
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Gene info
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Identity
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Gene
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Long gene namemab-21 like 2
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Synonyms gene name
- mab-21 (C. elegans)-like 2
- mab-21-like 2 (C. elegans)
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GenBank acession
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Locus
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Discovery year1999-07-06
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Entrez gene record
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RefSeq identity
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Classification
- MAB21 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemab-21 like 1
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Synonyms gene name
- mab-21 (C. elegans)-like 1
- mab-21-like 1 (C. elegans)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-08-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MAB21 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemab-21 like 3
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Synonyms gene
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Synonyms gene name
- chromosome 1 open reading frame 161
- mab-21-like 3 (C. elegans)
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MAB21 family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data