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Products_type
Antibody
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Products_short_name
[Kappa Light Chain, F(ab')2]
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Products_name_syn
[Anti -Kappa Light Chain, F(ab')2 (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) (Biotin)]
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Clonality
Polyclonal
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Reactivity
Human
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Specificity
Recognizes human kappa light chains.
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Purity
Purified by affinity chromatography.
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Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled to Biotin.
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Concentration
0.5mg/ml
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Storage_stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested_application
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF) - Other applications not tested.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Group
f(ab')2s
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Gene target
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Gene symbol
IGKC
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Short name
Kappa Light Chain, F(ab')2 (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) ( )
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Technique
F(ab')2, light, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph.
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Host
Rabbit
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Isotype
IgG F(ab')2, F(ab')2 Fragment Affinity-Purified Secondary Antibodies
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Label
Biotin
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Alternative name
Kappa Light epitope, F(Antibody')2 (HCAK1, Ig kappa epitope C Region, immunoglobulin kappa constant, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light epitope, Kappa 1 Immunoglobulin Light epitope, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) (biotinilated)
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Alternative technique
secondairy
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Alternative to gene target
immunoglobulin kappa constant, IGKC and IDBG-123549 and ENSG00000211592 and 102724332, protein binding, Extracellular, Igkc and IDBG-222519 and ENSMUSG00000076609 and, IGKC and IDBG-634738 and ENSBTAG00000003408 and
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Tissue
region
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