IgG2b Negative Control (Biotin)

• Catalog number
  MBS655641
• Price
  Please ask
• Size
  100 Tests

• Products_type
  Isotype Control
• Products_short_name
  [IgG2b Negative Control]
• Clone
  [3H2413]
• Specificity
  Can be used as a negative control for direct FITC immunofluorescence staining (eg. Flow Cytometry) when using mouse monoclonal antibodies to human cell surface antigens of isotype IgG2b. This enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections.
• Purity
  Affinity Purified Purified by Protein A affinity chromatography.
• Form
  Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
• Storage_stability
  -20 degree C
• Tested_application
  Flow Cytometry (FC/FACS)
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.

• Gene target
  IgG2b  
• Short name
  IgG2b Negative Control ( )
• Technique
  Negative, Control, controls, negatif
• Host
  Mouse
• Isotype
  IgG2b, IgG2b
• Label
  Biotin
• Alternative name
  IgG2b Negative reference (biotinilated)
• Alternative technique
  controls
• Tissue
  control

MeSH Data

• Name
  Negative Staining
• Concept
  Scope note: The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
• Tree numbers
  • E01.370.225.500.620.670.520
  • E01.370.225.750.600.670.520
  • E05.200.500.620.670.520
  • E05.200.750.600.670.520
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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