IgG1 Murine Negative Control (For rat tissue) (FITC)

• Catalog number
  MBS655692
• Price
  Please ask
• Size
  5x0.1 mg

• Products_type
  Isotype Control
• Products_short_name
  [IgG1 Murine Negative Control]
• Clone
  [3H1421]
• Reactivity
  Human, Rat
• Specificity
  Recognizes human CD45RA antigen. Note: Use with rat tissues only.
• Purity
  Affinity Purified Purified by Protein G affinity chromatography.
• Form
  Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  Flow Cytometry (FC/FACS)
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species. 6
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  IgG1   Murine   For   tissue  
• Short name
  IgG1 Murine Negative Control (For tissue) (FITC)
• Technique
  Negative, Control, tissue, FITC, controls, Fluorescein, negatif, tissues
• Host
  Tissue culture supernatant
• Isotype
  IgG1, IgG1
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  IgG1 Murine Negative reference (to measure rat tissue) (fluorecein)
• Alternative technique
  controls, tissues, fluorescine
• Tissue
  control, tissue

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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