IgG1 FITC / IgG1 RPE / IgG1 RPE-Cy5[IgG1 / IgG1 RPE / IgG1 RPE-Cy5]

Products_type

Antibody
Reactivity

Human
Properties

Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Cy5.5, Anti-FITC Antibody

Gene target

IgG1 IgG1 RPE IgG1 RPE [IgG1 IgG1 RPE IgG1 RPE
Gene symbol

RPE
Short name

IgG1 FITC / IgG1 RPE / IgG1 RPE- [IgG1 / IgG1 RPE / IgG1 RPE- ]
Technique

FITC, Fluorescein
Host

Mouse
Isotype

IgG1
Label

FITC
Alternative name

IgG1 fluorecein / IgG1 RPE / IgG1 RPE-cyanine 5[IgG1 / IgG1 RPE / IgG1 RPE-cyanine 5]
Alternative technique

fluorescine

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data