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Products_type
Immunoglobulin
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Products_short_name
[IgG, Sheep]
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Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep IgG and anti-sheep serum.
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Purity
Highly Purified Purified by salt fractionation and ion exchange chromatography.
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Form
Supplied as a lyophilized powder from PBS, pH 7.4, 15mg/ml BSA (IgG and Protease free). No preservative added. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
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Storage_stability
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.