IgG, H&L, X-Adsorbed (HRP)
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Catalog numberMBS624312
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Price:
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Size0.5 mg
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Products_typeAntibody
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Products_short_name[IgG, H&L, X-Adsorbed]
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Products_name_syn[Anti -IgG, H&L, X-Adsorbed (HRP)]
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ClonalityPolyclonal
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ReactivityRabbit
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SpecificityRecognizes heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on IEP. Does not recognize non-immunoglobulin rabbit serum proteins. Does not recognize serum proteins from bovine, human, or mouse or IgG from human or mouse.
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PurityAffinity Purified ~90% based on SDS-PAGE, may contain small amounts of intact IgG.
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FormSupplied as a lyophilized powder in 10mM sodium phosphate, 0.15M sodium chloride, pH7.2, 1% BSA, Protease/IgG free, 0.1% Kathon CG. Labeled with horseradish peroxidase (HRP).
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Storage_stabilityLyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
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Tested_applicationELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
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PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationHRP Conjugated
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short nameIgG, H L, X-Adsorbed ( )
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TechniqueIgG, IgGs
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HostGoat
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IsotypeF(ab')2, Heavy & Light chains, IgG
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LabelHRP
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Alternative nameImmunoglobulin G, heavy + light chain, X-Adsorbed (horseradish peroxidase)
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Alternative techniqueimmunoglobulins
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