IgG, H&L, X-Adsorbed (FITC)
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Catalog number
MBS614967
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Price
Please ask
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Size
1 mg
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Products_type
Antibody
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Products_short_name
[IgG, H&L, X-Adsorbed]
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Products_name_syn
[Anti -IgG, H&L, X-Adsorbed (FITC)]
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Clonality
Polyclonal
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Reactivity
Rat
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Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
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Purity
Affinity Purified Purified by immunoaffinity chromatography.
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Form
Supplied as alyophilized powder in PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (MW
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Storage_stability
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short name
IgG, H L, X-Adsorbed (FITC)
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Technique
FITC, IgG, Fluorescein, IgGs
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Host
Donkey
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Isotype
IgG, Heavy & Light chains, IgG
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Label
FITC
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Alternative name
Immunoglobulin G, heavy + light chain, X-Adsorbed (fluorecein)
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Alternative technique
fluorescine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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