IgG, H&L, X-adsorbed (Biotin)

  • Catalog number
    MBS618918
  • Price
    3426.24 USD
  • Size
    5x1 mg
  • Products_type
    Antibody
  • Products_short_name
    [IgG, H&L, X-adsorbed]
  • Products_name_syn
    [Anti -IgG, H&L, X-adsorbed (Biotin)]
  • Clonality
    Polyclonal
  • Reactivity
    Rabbit
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,
  • Purity
    Affinity Purified Purified by immunoaffinity chromatography.
  • Form
    Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per guinea pig IgG mol
  • Storage_stability
    Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
  • Tested_application
    ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Gene target
  • Short name
    IgG, H L, X-adsorbed ( )
  • Technique
    IgG, IgGs
  • Host
    Guinea pig
  • Isotype
    IgG, Heavy & Light chains, IgG
  • Label
    Biotin
  • Alternative name
    Immunoglobulin G, heavy + light chain, X-adsorbed (biotinilated)
  • Alternative technique
    immunoglobulins
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