IgG, Hamster Isotype Control (Biotin)
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Catalog numberMBS655743
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Price:
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Size5x0.5 mg
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Products_typeIsotype Control
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Products_short_name[IgG, Hamster Isotype Control]
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PurityPurified Purified
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FormSupplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
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Storage_stabilityMay be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_applicationELISA (EL/EIA), Flow Cytometry (FC/FACS)
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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ConjugationBiotinylated
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DescriptionIsotype or positive controls by peptides, antibodies and deactivated samples.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Groupisotypes
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Short nameIgG, Isotype Control ( )
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TechniqueControl, Isotype, IgG, controls, IgGs, Isotypes and controls
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HostPool of normal Syrian hamster sera.
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IsotypeIgG, IgG
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LabelBiotin
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SpeciesHamster, Hamsters
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Alternative nameImmunoglobulin G, Hamster isoform reference (biotinilated)
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Alternative techniquecontrol, controls, immunoglobulins
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Tissuecontrol
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