IgG, Fc, X-Adsorbed (FITC)

• Catalog number
  MBS626495
• Price
  2238.01 USD
• Size
  5x0.5 mg

• Products_type
  Antibody
• Products_short_name
  [IgG, Fc, X-Adsorbed]
• Products_name_syn
  [Anti -IgG, Fc, X-Adsorbed (FITC)]
• Clonality
  Polyclonal
• Reactivity
  Human
• Specificity
  Recognizes heavy chains on human IgG based on IEP. Minimum crossreactivity with bovine, horse, mouse or rabbit serum proteins.
• Purity
  Affinity Purified ~90% based on SDS-PAGE., small amounts of intact IgG may be present.
• Form
  Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
• Tested_application
  Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  X-Adsorbed  
• Short name
  IgG, Fc, X-Adsorbed (FITC)
• Technique
  FITC, IgG, FC, Fluorescein, IgGs
• Host
  Goat
• Isotype
  F(ab')2, IgG
• Label
  FITC
• Alternative name
  Immunoglobulin G, fragment c, X-Adsorbed (fluorecein)
• Alternative technique
  fluorescine, immunoglobulins

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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