IgG, Fc, X-adsorbed (Biotin)
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Catalog number
MBS616897
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Price
2970.03 USD
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Size
5x0.5 mg
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Products_type
Antibody
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Products_short_name
[IgG, Fc, X-adsorbed]
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Products_name_syn
[Anti -IgG, Fc, X-adsorbed (Biotin)]
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Clonality
Polyclonal
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Reactivity
Mouse
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Specificity
Recognizes Mouse IgG F(c) fragment. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgG, Mouse IgG F(c) and mouse serum. Species Crossreactivity: Does not crossreact with anti-pepsin, anti-goat IgG F(c), mouse IgG F(ab')2 or bovine, equine and human serum proteins.
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Purity
Affinity Purified Purified by immunoaffinity chromatography.
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Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin.
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Storage_stability
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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