IgG, Fc, X-Adsorbed (Biotin)
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Catalog number
MBS625544
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Price
585.17 USD
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Size
0.5 mg
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Products_type
Antibody
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Products_short_name
[IgG, Fc, X-Adsorbed]
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Products_name_syn
[Anti -IgG, Fc, X-Adsorbed (Biotin)]
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Clonality
Polyclonal
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Reactivity
Human
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Specificity
Recognizes heavy chains on human IgG based on IEP. Minimum crossreactivity with bovine, horse, mouse or rabbit serum proteins.
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Purity
Affinity Purified ~90% based on SDS-PAGE, may contain small amounts of intact IgG.
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Form
Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Biotin.
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Storage_stability
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested_application
ELISA (EL/EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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