IgG F(ab')2, X-adsorbed (Biotin)

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• Catalog number
  MBS619512
• Price:

  779.58 USD

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• Size
  0.5 mg

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• Products_type
  Antibody
• Products_short_name
  [IgG F(ab')2, X-adsorbed]
• Products_name_syn
  [Anti -IgG F(ab')2, X-adsorbed (Biotin)]
• Clonality
  Polyclonal
• Reactivity
  Rat
• Specificity
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
• Purity
  Affinity Purified Purified by immunoaffinity chromatography.
• Form
  Supplied as a lyophilized powder in PBS, pH 7.2, 15mg/ml BSA (IgG and Protease free), 0.05% sodium azide. Reconstitution: Reconstitute with 500ul sterile dH2O.
• Storage_stability
  Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Group
  f(ab')2s
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

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• Gene target
  X-adsorbed  
• Short name
  IgG F(ab')2, X-adsorbed ( )
• Technique
  F(ab')2, IgG,  F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. IgGs
• Host
  Goat
• Isotype
  F(ab')2, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
• Label
  Biotin
• Alternative name
  Immunoglobulin G F(Antibody')2, X-adsorbed (biotinilated)
• Alternative technique
  secondairy, immunoglobulins

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