IgG, F(ab')2 (HRP)
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Catalog numberMBS621777
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Price:
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Size5x0.5 mg
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Products_typeAntibody
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Products_short_name[IgG, F(ab')2]
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Products_name_syn[Anti -IgG, F(ab')2 (HRP)]
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ClonalityPolyclonal
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ReactivityRabbit
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SpecificityRecognizes rabbit IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rabbit IgG, rabbit IgG F(ab')2 and rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), rabbit IgG F(c), bovine, equine, human, mouse, rat or sheep serum proteins.
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PurityAffinity Purified Purified by immunoaffinity chromatography.
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FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free) 0.01% Gentamicin Sulfate. Labeled with HRP (MW: 40kD). Reconstitute with 500ul sterile ddH2O.
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Storage_stabilityLyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
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Tested_applicationELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
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Groupf(ab')2s
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PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationHRP Conjugated
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Short nameIgG, F(ab')2 ( )
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TechniqueF(ab')2, IgG, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. IgGs
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HostGoat
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IsotypeF(ab')2, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
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LabelHRP
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Alternative nameImmunoglobulin G, F(Antibody')2 (horseradish peroxidase)
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Alternative techniquesecondairy, immunoglobulins
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