Iduronidase, a-L-, Recombinant, Human (a-L-Iduronidase, IDUA)

Products_type

Active Protein
Products_short_name

[Iduronidase, a-L-]
Other_names

[iduronidase; Alpha-L-iduronidase; alpha-L-iduronidase; iduronidase, alpha-L-]
Other_gene_names

[Idua; Idua; 6030426D08]
Purity

Highly Purified~ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
Form

Supplied as a liquid in 50mM sodium acetate, 500mM sodium chloride, pH 5.0.
Storage_stability

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Iduronidase a-L a-L-Iduronidase IDUA
Gene symbol

CPT1A, POM121L2, L3MBTL2, CLDN10, RPL32P1, FABP1, COX7A2, NFIA, ATRN, ANP32E
Short name

Iduronidase, a-L-, Recombinant, (a-L-Iduronidase, IDUA)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

NSO cells
Species

Human, Humans
Alternative name

Iduronidase, a-L-, Rec., H. sapiens (a-L-Iduronidase, IDUA)
Alternative technique

rec

Identity

HGNC:13973
Gene

POM121L2
Long gene name

POM121 transmembrane nucleoporin like 2
Synonyms gene name
- POM121 membrane glycoprotein-like 2 (rat)
- POM121 membrane glycoprotein-like 2
- POM121 transmembrane nucleoporin-like 2
Synonyms
- POM121-L
GenBank acession
- AL021808
Locus

6p22.1
Discovery year

2001-07-20
Entrez gene record

94026
RefSeq identity
- NM_033482
VEGA ID

OTTHUMG00000014475

Identity

HGNC:18594
Gene

L3MBTL2
Long gene name

L3MBTL histone methyl-lysine binding protein 2
Synonyms gene name
- l(3)mbt-like 2 (Drosophila)
- L3MBTL2, polycomb repressive complex 1 subunit
Synonyms
GenBank acession
- AJ305226
Locus

22q13.2
Discovery year

2002-04-29
Entrez gene record

83746
Pubmed identfication
- 11682070
- 21596310
RefSeq identity
- NM_031488
Classification
- MBT domain containing
VEGA ID

OTTHUMG00000150942

Identity

HGNC:16673
Gene

ANP32E
Long gene name

acidic nuclear phosphoprotein 32 family member E
Synonyms gene name
- acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Synonyms
- LANPL
- MGC5350
- LANP-L
GenBank acession
- AK092672
Locus

1q21.2
Discovery year

2003-04-15
Entrez gene record

81611
Pubmed identfication
- 12438741
RefSeq identity
- NM_030920
Classification
- ANP32 acidic nuclear phosphoproteins
VEGA ID

OTTHUMG00000012547

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data