Human Perforin ELISpot Set

Products_type

ELISpot Set
Products_short_name

[Perforin]
Products_name_syn

[Perforin (with non-sterile plate)]
Other_names

[perforin; Perforin-1; perforin-1; perforin 1 (pore forming protein); Cytolysin; Lymphocyte pore-forming protein; PFP]
Other_gene_names

[PRF1; PRF1; P1; PFP; FLH2; PFN1; HPLH2; PFP; P1; PFP]
Reactivity

Human
Specificity

The assay recognizes natural human Perforin. To define the specificity several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (Granzyme B, Fas, Fas L, IL-1beta, IL-2, IFNgamma, TNFalpha and TRAIL). This testing was performed using the equivalent human Perforin antibody pair in an ELISA assay.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Name

Enzyme-Linked Immunospot Assay
Concept

Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
Qualifiers

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