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Products_type
Active Protein
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Products_short_name
[leptin N82K mutant]
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Products_name_syn
[Human leptin N82K mutant LEP-15]
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Purity
Purity of recombinant human leptin N82K mutant is greater than 98.0% as determined by:(a) Gel filtration analysis of the recombinant protein.(b) Analysis by reducing and non-reducing SDS-PAGE gel.
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Form
White lyophilized (freeze-dried) powder.
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Storage_stability
Lyophilized recombinant human leptin N82K mutant although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 mg human leptin per ml and up to 2 mg and filter sterilization recombinant human leptin N82K mutant can be stored at +4 degree C or even room temperature for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
