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Products_type
Isotype Control
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Products_short_name
[Human IgG1 kappa Isotype Control]
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Products_name_syn
[Human IgG1 Kappa - HRP; Human IgG1 kappa Isotype Control]
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Purity
Purity: >95% as judged by SDS-polyacrylamide gel electrophoresis. Purification: Affinity chromatography on protein G covalently linked to agarose
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Form
Horseradish Peroxidase (HRP) Conjugate
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Storage_stability
The purified (UNLB) IgG is provided at a concentration of 0.5 mg/mL in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. The human IgG solution has been centrifuged and passed through a 0.22 u filter, and is free of any visible precipitate. The product should be stored at 2-8 degree C and is stable for the period shown on the label if stored as directed. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
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Tested_application
ELISA; Immunoblot; SDS-Page
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
