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Products_type
Isotype Control
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Products_short_name
[Human IgG Isotype Control]
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Products_name_syn
[Human IgG - HRP; Human IgG Isotype Control]
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Purity
Purity: >95% as judged by SDS-polyacrylamide gel electrophoresis Purification: Affinity chromatography on protein G covalently linked to agarose
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Form
Horseradish Peroxidase (HRP) Conjugate
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Storage_stability
The purified (UNLB) IgG is provided at a concentration of 0.5 mg/mL in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. The human IgG solution has been centrifuged and passed through a 0.22 u filter, and is free of any visible precipitate. The product should be stored at 2-8 degree C and is stable for the period shown on the label if stored as directed. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
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Tested_application
ELISA; Immunoblot; SDS-Page
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
