Horse IgG Whole Molecule-Biotin Conjugate
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Catalog number
MBS689584
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Price
Please ask
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Size
5x1 mg
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Products_type
Immunoglobulin
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Products_short_name
[IgG]
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Products_name_syn
[Horse IgG-Biotin Conjugate]
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Purity
> 95% based on SDS-PAGE Purified.
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Form
Liquid
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Concentration
1.5 mg/ml
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Storage_stability
Store at 2-8 degree C
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Tested_application
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
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Conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Description
Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
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