HLA Class 1 Antigen B12 (FITC)

  • Catalog number
    MBS600362
  • Price
    Please ask
  • Size
    0.25 mL
  • Products_type
    Antibody
  • Products_short_name
    [HLA Class 1 Antigen B12]
  • Products_name_syn
    [Anti -HLA Class 1 Antigen B12 (FITC)]
  • Other_names
    [HLA class I histocompatibility antigen, B-40 alpha chain; HLA class I histocompatibility antigen, B-7 alpha chain; major histocompatibility complex, class I, B; lymphocyte antigen; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC class I antigen GN00104; leukocyte antigen class I-B; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen HLA-B heavy chain; HLA class I histocompatibility antigen, B alpha chain; major histocompatibility complex, class I, B; MHC class I antigen B*7]
  • Other_gene_names
    [HLA-B; HLA-B; AS; HLAB; SPDA1; HLAB]
  • Clonality
    Monoclonal
  • Clone
    [0.L.4]
  • Reactivity
    Human
  • Specificity
    Recognizes human HLA Class 1 Antigen-B12.Sprecifity was determined at a 1:10 dilution bye the microcytotoxicity test under standard NIH conditions.
  • Purity
    Highly Purified Purified by Ion Exchange chromatography.
  • Form
    Supplied as a liquid in PBS, 0.09% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (FITC).
  • Storage_stability
    Store product at 4°C if to be used inmediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
  • Tested_application
    Flow Cytometry (FC/FACS).
  • Description
    Antigens are peptides or recombinant or native dependent on the production method.
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Gene target
    HLA   Class   B12  
  • Gene symbol
    NDUFB3, HLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DQA2, HLA-DPB1, HLA-DQB2, HLA-W, HLA-DRA
  • Short name
    HLA Class 1 Antigen B12 (FITC)
  • Technique
    antigen, FITC, antigenes, Fluorescein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Label
    FITC
  • Alternative name
    Human leukocyte antigen Class 1 protein B12 (fluorecein)
  • Alternative technique
    antigenes, fluorescine
Gene info
  • Identity
    HGNC:7698
  • Gene
    NDUFB3
  • Long gene name
    NADH:ubiquinone oxidoreductase subunit B3
  • Synonyms gene name
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3 (12kD, B12)
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa
  • Synonyms
  • Synonyms name
  • GenBank acession
  • Locus
    2q33.1
  • Discovery year
    1997-12-17
  • Entrez gene record
    4709
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • NADH:ubiquinone oxidoreductase supernumerary subunits
  • VEGA ID
    OTTHUMG00000132820
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
    Fluorescent Antibody Technique
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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