HL60 (HL-60, Leukemia Cell Line HL-60)

Products_type

Antibody
Products_short_name

[HL60]
Products_name_syn

[Anti -HL60 (HL-60, Leukemia Cell Line HL-60)]
Clonality

Monoclonal
Clone

[IPO-M6]
Reactivity

Human
Specificity

Recognizes human leukemia cell line HL-60. Immunoprecipitates two proteins of MW of 48 and 52kD. Does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 or T-cell lines CCRF-HSB2, Jurkat and Molt-4.
Purity

Purified Purified
Form

Supplied as a liquid in PBS, 0.05% sodium azide.
Storage_stability

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Tested_application

Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

HL60 HL-60 Leukemia Line HL-60
Gene symbol

HMGCL, IGLV4-60, IGHVII-60-1, IGHV3-60, RCC2, LIPC, ITLN2, OGFR, RNU6-60P, SPINK4
Short name

HL60 (HL-60, Leukemia Line HL-60)
Host

Mouse
Isotype

IgG1
Alternative name

HL60 (HL-60, Leukemia cellular Line HL-60)
Tissue

cell
Virus

leukemia

Identity

HGNC:5005
Gene

HMGCL
Long gene name

3-hydroxy-3-methylglutaryl-CoA lyase
Synonyms gene name
- 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
- 3-hydroxymethyl-3-methylglutaryl-CoA lyase
Synonyms
- HL
Synonyms name
- hydroxymethylglutaricaciduria
- hydroxymethylglutaryl-CoA lyase
GenBank acession
- BC010570
Locus

1p36.11
Discovery year

1993-12-13
Entrez gene record

3155
Pubmed identfication
- 8102917
- 8978493
RefSeq identity
- NM_000191
VEGA ID

OTTHUMG00000002963

Identity

HGNC:5686
Gene

IGHVII-60-1
Long gene name

immunoglobulin heavy variable (II)-60-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-60-1
- immunoglobulin heavy variable (II)-60-1 pseudogene
Synonyms
- IGHV(II)-60-1
GenBank acession
- AB019437
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28361
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151951

Identity

HGNC:5614
Gene

IGHV3-60
Long gene name

immunoglobulin heavy variable 3-60 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable 3-60
- immunoglobulin heavy variable 3-60 pseudogene
GenBank acession
- M29813
Locus

14q32.33
Discovery year

2000-04-04
Entrez gene record

28417
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151956

Name

Leukemia, Experimental
Concept

Scope note: Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
Tree numbers
- C04.557.337.372
- C04.619.531
- E05.598.500.496.500
Qualifiers

diagnosis, parasitology, immunology, microbiology, diagnostic imaging, physiopathology, rehabilitation, enzymology, etiology, mortality, epidemiology, blood, cerebrospinal fluid, chemically induced, congenital, diet therapy, drug therapy, therapy, psychology, urine, virology, history, classification, economics, embryology, genetics, nursing, radiotherapy, ethnology, prevention & control, pathology, metabolism, complications, surgery