Heartworm, Canine (Dirofilaria immitis) (Biotin)

Products_type

Antibody
Products_short_name

[Heartworm, Canine]
Products_name_syn

[Anti -Heartworm, Canine (Dirofilaria immitis) (Biotin)]
Clonality

Polyclonal
Specificity

Recognizes soluble & structural antigens of the adult worm. Antiserum is not absorbed for related filarial Nematodes. Crossreactivity: Does not react with canine serum.
Purity

Highly Purified Purified IgG fraction to a purity of > 95%.
Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added. Conjugated to Biotin.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Gene target

Heartworm Dirofilaria immitis
Short name

Heartworm, (Dirofilaria immitis) ( )
Host

Goat
Isotype

IgG
Label

Biotin
Species

From Canis lupus or the domestic dog we know mostly the Beagle to be used in drug discovery experiments.
Alternative name

Heartworm, canine (Dirofilaria immitis) (biotinilated)

Name

Radioimmunosorbent Test
Concept

Scope note: Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
Tree numbers
- E05.478.566.380.830
- E05.478.566.639.830
- E05.601.470.380.830
- E05.601.470.639.830
Qualifiers

ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data