Hamster Anti-Mouse CD81-FITC
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Catalog number
MBS673605
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Price
Please ask
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Size
5x0.5 mg
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Products_type
Antibody
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Products_short_name
[CD81/TAPA-1]
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Products_name_syn
[Hamster Anti-Mouse CD81 - FITC; Hamster Anti-Mouse CD81/TAPA-1]
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Other_names
[CD81 antigen; CD81 antigen; CD81 antigen; tspan-28; tetraspanin-28; OTTHUMP00000011528; OTTHUMP00000011529; OTTHUMP00000229004; OTTHUMP00000229005; OTTHUMP00000229006; OTTHUMP00000229008; OTTHUMP00000229358; OTTHUMP00000229392; 26 kDa cell surface protein TAPA-1; CD81 antigen (target of antiproliferative antibody 1); CD81 molecule; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28]
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Other_gene_names
[CD81; CD81; S5.7; CVID6; TAPA1; TSPAN28; TAPA1; TSPAN28]
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Clonality
Monoclonal
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Clone
[2F7]
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Reactivity
Mouse
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Specificity
Mouse CD81 (Mr 25 kDa), a member of the transmembrane 4 integral membrane protein family CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse.1 CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors.2 Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha.2 The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro.2
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Form
Fluorescein (FITC) Conjugate
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Storage_stability
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested_application
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD81-AS1, CD81
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Short name
Anti-Mouse CD81-FITC
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Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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Host
Armenian Hamster
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Isotype
Armenian Hamster IgG, group 3, kappa
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Label
FITC
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Species
Mouse, Hamsters, Mouses
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Alternative name
Hamster Antibody toMouse CD81 molecule-fluorecein
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD81 molecule, CVID6 and S5.7 and TAPA1 and TSPAN28, CD81 and IDBG-22455 and ENSG00000110651 and 975, MHC class II protein complex binding, Cell surfaces, Cd81 and IDBG-212677 and ENSMUSG00000037706 and 12520, CD81 and IDBG-645214 and ENSBTAG00000047495 and 511435
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Gene info
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Identity
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Gene
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Long gene name
CD81 antisense RNA 1
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GenBank acession
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Locus
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Discovery year
2013-10-18
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Entrez gene record
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
CD81 molecule
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Synonyms gene
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Synonyms gene name
- CD81 antigen (target of antiproliferative antibody 1)
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Synonyms
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Locus
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Discovery year
1992-10-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tetraspanins
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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