Hamster Anti-Mouse CD3e-CY5

• Catalog number
  MBS673144
• Price
  Please ask
• Size
  5x0.1 mg

• Products_type
  Antibody
• Products_short_name
  [CD3 epsilon]
• Products_name_syn
  [Hamster Anti-Mouse CD3e - Cyanine5-Labeled; Hamster Anti-Mouse CD3 epsilon]
• Other_names
  [CD3 epsilon; CD3 epsilon]
• Other_gene_names
  [cd3e]
• Clonality
  Monoclonal
• Clone
  [500-A2]
• Reactivity
  Mouse
• Specificity
  Mouse CD3 epsilon, Mr 25 kDa CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 epsilon is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. 500-A2 recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex of mouse strains tested.^1, 2, 3
• Form
  Cyanine 5 (CY5) Conjugate
• Storage_stability
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) and Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested_application
  Flow Cytometry; Immunoprecipitation
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
• Conjugation
  Cy5.5
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD3e  
• Gene symbol
  CD3E, POLR1G
• Short name
  Anti-Mouse CD3e-
• Technique
  anti-, Mouse, anti, antibody to, antibodies, mouses
• Host
  Syrian Hamster
• Isotype
  Syrian Hamster IgG kappa
• Label
  Cy5
• Species
  Mouse, Hamsters, Mouses
• Alternative name
  Hamster Antibody toMouse CD3e molecule, epsilon (CD3-TCR complex)-cyanine 5
• Alternative technique
  murine, antibodies
• Alternative to gene target
  CD3e molecule, epsilon (CD3-TCR complex), IMD18 and T3E and TCRE, CD3E and IDBG-73617 and ENSG00000198851 and 916, protein heterodimerization activity, Cell surfaces, Cd3e and IDBG-159085 and ENSMUSG00000032093 and 12501, CD3E and IDBG-631260 and ENSBTAG00000015710 and 281054

Gene info

• Identity
  HGNC:1674
• Gene
  CD3E
• Long gene name
  CD3e molecule
• Synonyms gene name
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
• GenBank acession
  • X03884
• Locus
  11q23.3
• Discovery year
  1986-01-01
• Entrez gene record
  916
• RefSeq identity
  • NM_000733
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000166968
• Locus Specific Databases
  • CD3Ebase: Mutation registry for autosomal recessive CD3epsilon immunodeficiency
  • LRG_38

Gene info

• Identity
  HGNC:24219
• Gene
  POLR1G
• Long gene name
  RNA polymerase I subunit G
• Synonyms gene
  • CD3EAP
• Synonyms gene name
  • CD3e antigen, epsilon polypeptide associated protein
  • CD3e molecule associated protein
• Synonyms
  • ASE-1
  • CAST
  • PAF49
  • RPA34
  • A34.5
• Synonyms name
  • CD3 epsilon associated protein
  • antisense to ERCC 1
• GenBank acession
  • U86751
• Locus
  19q13.32
• Discovery year
  2005-04-21
• Entrez gene record
  10849
• Pubmed identfication
  • 10373416
  • 9426281
  • 15226435
  • 23150253
• RefSeq identity
  • NM_012099
• Classification
  • RNA polymerase subunits
• VEGA ID
  OTTHUMG00000182120

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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