Hamster Anti-Mouse CD154/CD40L-LE/AF

• Catalog number
  MBS673365
• Price
  Please ask
• Size
  0.5 mg

• Products_type
  Antibody
• Products_short_name
  [CD154]
• Products_name_syn
  [Hamster Anti-Mouse CD154/CD40L - Low Endotoxin/Azide-Free; Hamster Anti-Mouse CD154]
• Clonality
  Monoclonal
• Clone
  [MR1]
• Reactivity
  Mouse
• Specificity
  CD154 (Mouse CD40 ligand/gp39), Mr 39-kDa CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands.^1-7 It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4^+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporinsensitive. 6 The MR1 monoclonal antibody binds to murine CD154 with high affinity, blocks binding to CD40, and blocks CD154 function.^1, 5 Administration of this antibody to mice blocks the ability to mount primary and secondary immune responses to TD antigens, yet does not alter the immune response to TI antigens.^4
• Form
  Low Endotoxin, Azide - Free (LE/AF)
• Storage_stability
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested_application
  Flow Cytometry; Immunoprecipitation
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD154/CD40L-LE/AF  
• Gene symbol
  CD40LG, FUT3, HPS4, NPAS4
• Short name
  Anti-Mouse CD154/CD40L-LE/AF
• Technique
  anti-, Mouse, anti, antibody to, antibodies, mouses
• Host
  Armenian Hamster
• Isotype
  Armenian Hamster IgG kappa
• Species
  Mouse, Hamsters, Mouses
• Alternative name
  Hamster Antibody toMouse CD154/CD40L-LE/AF
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:11935
• Gene
  CD40LG
• Long gene name
  CD40 ligand
• Synonyms gene
  • HIGM1
  • IMD3
  • TNFSF5
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
• Synonyms
  • CD40L
  • TRAP
  • gp39
  • hCD40L
  • CD154
• Synonyms name
  • CD40 antigen ligand
  • tumor necrosis factor (ligand) superfamily member 5
  • T-B cell-activating molecule
  • TNF-related activation protein
  • hyper-IgM syndrome
• GenBank acession
  • X67878
• Locus
  Xq26.3
• Discovery year
  1989-06-30
• Entrez gene record
  959
• Pubmed identfication
  • 1427881
  • 7678782
• RefSeq identity
  • NM_000074
• Classification
  • CD molecules
  • Tumor necrosis factor superfamily
• VEGA ID
  OTTHUMG00000022512
• Locus Specific Databases
  • CD40Lbase: Mutation registry for X-linked Hyper-IgM syndrome
  • Global Variome shared LOVD
  • LRG_141

Gene info

• Identity
  HGNC:4014
• Gene
  FUT3
• Long gene name
  fucosyltransferase 3 (Lewis blood group)
• Synonyms gene
  • LE
• Synonyms gene name
  • fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included)
• Synonyms
  • CD174
• Synonyms name
  • galactoside 3(4)-L-fucosyltransferase
• Locus
  19p13.3
• Discovery year
  1993-04-08
• Entrez gene record
  2525
• Pubmed identfication
  • 1977660
  • 1740457
• RefSeq identity
  • NM_000149
• Classification
  • Fucosyltransferases
  • Blood group antigens
  • CD molecules
• VEGA ID
  OTTHUMG00000180614
• Locus Specific Databases
  • Blood Group Antigen Mutation Database
  • LRG_800

Gene info

• Identity
  HGNC:15844
• Gene
  HPS4
• Long gene name
  HPS4 biogenesis of lysosomal organelles complex 3 subunit 2
• Synonyms gene name
  • Hermansky-Pudlak syndrome 4
• Synonyms
  • KIAA1667
  • LE
  • BLOC3S2
• Locus
  22q12.1
• Discovery year
  2001-06-28
• Entrez gene record
  89781
• Pubmed identfication
  • 11836498
  • 12663659
• RefSeq identity
  • NM_022081
• Classification
  • Biogenesis of lysosomal organelles complex 3
• VEGA ID
  OTTHUMG00000030459
• Locus Specific Databases
  • Mutations of the Human light ear Gene (le, HPS4)
  • LRG_590

Gene info

• Identity
  HGNC:18983
• Gene
  NPAS4
• Long gene name
  neuronal PAS domain protein 4
• Synonyms
  • PASD10
  • NXF
  • Le-PAS
  • bHLHe79
• GenBank acession
  • AB049469
• Locus
  11q13.2
• Discovery year
  2005-09-26
• Entrez gene record
  266743
• Pubmed identfication
  • 14701734
• RefSeq identity
  • NM_178864
• Classification
  • Basic helix-loop-helix proteins
• VEGA ID
  OTTHUMG00000167045

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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