Griffonia (Bandeiraea) simplicifolia GS II (GS-II) (FITC)

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Properties

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Gene target

Griffonia Bandeiraea simplicifolia GS-II
Gene symbol

ABCC1, SPINK2, CRABP2, MGST2, IBSP, APOL2, MGAT2, P4HA2, XYLT2, TMED10P1
Short name

Griffonia (Bandeiraea) simplicifolia GS II (GS-II) (FITC)
Technique

FITC, Fluorescein
Label

FITC
Alternative name

Griffonia (Bandeiraea) simplicifolia GS II (GS-II) (fluorecein)
Alternative technique

fluorescine

Identity

HGNC:51
Gene

ABCC1
Long gene name

ATP binding cassette subfamily C member 1
Synonyms gene
- MRP
- MRP1
Synonyms gene name
- multidrug resistance associated protein 1
- ATP-binding cassette, sub-family C (CFTR/MRP), member 1
Synonyms
- GS-X
Synonyms name
- glutathione S-conjugate export pump
GenBank acession
- L05628
Locus

16p13.11
Discovery year

1993-06-29
Entrez gene record

4363
Pubmed identfication
- 8098549
- 1360704
RefSeq identity
- NM_004996
Classification
- ATP binding cassette subfamily C
VEGA ID

OTTHUMG00000048267

Identity

HGNC:11245
Gene

SPINK2
Long gene name

serine peptidase inhibitor Kazal type 2
Synonyms gene name
- serine protease inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
Synonyms
- HUSI-II
Synonyms name
- acrosin-trypsin inhibitor
GenBank acession
- BC022514
Locus

4q12
Discovery year

1993-12-01
Entrez gene record

6691
Pubmed identfication
- 8428671
RefSeq identity
- NM_021114
Classification
- Serine peptidase inhibitors, Kazal type
VEGA ID

OTTHUMG00000128769

Identity

HGNC:7045
Gene

MGAT2
Long gene name

alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Synonyms gene name
- mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Synonyms
- GNT-II
GenBank acession
- U15128
Locus

14q21.3
Discovery year

1993-02-16
Entrez gene record

4247
Pubmed identfication
- 7635144
RefSeq identity
- NM_002408
Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
VEGA ID

OTTHUMG00000140271
Locus Specific Databases
- Congenital Disorder of Glycosylation pages

Identity

HGNC:8547
Gene

P4HA2
Long gene name

prolyl 4-hydroxylase subunit alpha 2
Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
- prolyl 4-hydroxylase, alpha polypeptide II
Synonyms
- C-P4Halpha(II)
Synonyms name
- 4-PH alpha 2
- collagen prolyl 4-hydroxylase alpha(II)
GenBank acession
- U90441
Locus

5q31.1
Discovery year

1999-02-23
Entrez gene record

8974
Pubmed identfication
- 9211872
- 9149945
RefSeq identity
- NM_004199
Classification
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000059647

Identity

HGNC:30754
Gene

TMED10P1
Long gene name

transmembrane p24 trafficking protein 10 pseudogene 1
Synonyms gene
- TMED10P
Synonyms gene name
- transmembrane emp24-like trafficking protein 10 (yeast) pseudogene
- transmembrane emp24-like trafficking protein 10 (yeast) pseudogene 1
Synonyms
- Tmp21-II
GenBank acession
- AJ004914
Locus

8q24.3
Discovery year

2005-08-26
Entrez gene record

286102
Pubmed identfication
- 10376215
RefSeq identity
- XR_002807
VEGA ID

OTTHUMG00000165203

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data