GPCR GPR87 BIOTIN-Conjugated

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Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Identity

HGNC:4532
Gene

HCAR1
Long gene name

hydroxycarboxylic acid receptor 1
Synonyms gene
- GPR104
- GPR81
Synonyms gene name
- G protein-coupled receptor 104
- G protein-coupled receptor 81
Synonyms
- HCA1
- FKSG80
- TA-GPCR
- LACR1
Synonyms name
- lactate receptor 1
GenBank acession
- AF411110
Locus

12q24.31
Discovery year

2000-01-20
Entrez gene record

27198
Pubmed identfication
- 11574155
- 19047060
- 18952058
- 21454438
Classification
- Hydroxycarboxylic acid receptors

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

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