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Products_type
Secondary Antibody
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Products_short_name
[Goat F(ab')2 Anti-Mouse IgG (H+L)]
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Products_name_syn
[Goat F(ab')2 Anti-Mouse IgG(H+L), Human ads - Cyanine5-Labeled; Goat F(ab')2 Anti-Mouse IgG (H+L)]
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Clonality
Polyclonal
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Reactivity
Mouse
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Specificity
Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
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Purity
Gel filtration chromatography of pepsin digested antibody.
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Form
Cyanine 5 (CY5) Conjugate
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Storage_stability
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
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Tested_application
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Cy5.5
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Group
f(ab')2s
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Latin name
Capra aegagrus hircus, Mus musculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short name
Goat F(ab')2 Anti-Mouse IgG (H+L), ads-
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Technique
F(ab')2, anti-, Mouse, anti, Goat, IgG, antibody to, antibodies, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. goats, IgGs, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
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Label
Cy5
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Species
Human, Goats, Humans, Mouses
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Alternative name
caprine F(Antibody')2 Antibody toMouse Immunoglobulin G (heavy + light chain), H. sapiens ads-cyanine 5
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Alternative technique
secondairy, murine, antibodies, caprine, immunoglobulins
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