GOAT ANTI POLIOVIRUS 1/2/3:FITC
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Catalog number
MBS220337
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Price
Please ask
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Size
1 mL
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Products_type
Antibody
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Products_short_name
[POLIOVIRUS]
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Clonality
Polyclonal
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Form
FITC IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
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Concentration
IgG concentration 4 mg/ml
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Storage_stability
Store at 4 degree C, -20 degree C if preferred. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
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Tested_application
Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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Gene target
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Short name
GOAT ANTI POLIOVIRUS 1/2/3:FITC
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Technique
anti, FITC, Goat, antibody to, Fluorescein, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG
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Label
FITC
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Species
Goats
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Alternative name
caprine antibody to POLIOVIRUS 1/2/3:fluorecein
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Alternative technique
antibodies, caprine, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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