GOAT ANTI MOUSE LAMBDA LIGHT CHAIN:FITC
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Catalog number
MBS220260
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Price
Please ask
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Size
5x1 mg
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Products_type
Antibody
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Products_short_name
[LAMBDA LIGHT CHAIN]
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Other_names
[Ig lambda-2 chain C region, partial; Ig lambda-2 chain C region; immunoglobulin lambda constant 2]
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Products_gene_name
[LAMBDA]
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Other_gene_names
[Iglc2; Iglc2; Igl-C2]
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Clonality
Polyclonal
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Specificity
Goat anti Mouse Lambda Light Chain antibody recognizes mouse lambda 1 and lambda 2 light chains (free and bound) as demonstrated by ELISA. To minimize cross reactivity this product has been cross absorbed against mouse paraproteins with kappa light chains.
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Form
Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
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Concentration
Ig concentration 1.0 mg/ml
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Storage_stability
Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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Tested_application
Flow cytometry (FC/FACS), Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Mus musculus
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Gene symbol
FCGRT, CRYL1, TNFSF14
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Short name
GOAT ANTI MOUSE LAMBDA LIGHT CHAIN:FITC
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Technique
anti mouse, light, Mouse, anti, FITC, Goat, antibody to, anti mouse antibodies, Fluorescein, goats, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG
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Label
FITC
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Species
Mouse, Goats, Mouses
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Alternative name
caprine antibody to MOUSE LAMBDA LIGHT epitope:fluorecein
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Alternative technique
murine, antibodies, caprine, fluorescine
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Gene info
Gene info
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Identity
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Gene
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Long gene name
crystallin lambda 1
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Synonyms gene name
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2002-02-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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