Goat Anti-Mouse IgG2b Antibody, FITC Conjugated
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Catalog number
MBS191396
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Price
Please ask
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Size
1 mg
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Products_type
Antibody
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Products_short_name
[IgG2b]
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Products_name_syn
[Mouse IgG2b-FITC Conjugate]
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Other_names
[IgG2b, partial]
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Products_gene_name
[IgG2b]
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Specificity
Antibody is affinity-purified on mouse IgG2b. Cross-reactivity with mouse IgA, IgM, IgG1, IgG2a, IgG2c, IgG3, and IgE is not detectable.
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Purity
Affinity-purified
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Form
1mg/ml in phosphate-buffered saline (PBS), 0.2% BSA, 0.09% sodium azide, conjugated to fluorescein isothiocyanate. Fluorophore:antibody ratio = 5.7.
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Storage_stability
This antibody is stable for at least 1 year at 2-8 degree C.
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Tested_application
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Mus musculus
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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French translation
anticorps
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Gene target
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Short name
Goat Anti-Mouse IgG2b Antibody, FITC Conjugated
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Technique
Antibody, anti-, Mouse, anti, FITC, Goat, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, goats, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG2b
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Label
FITC
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Species
Mouse, Goats, Mouses
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Alternative name
caprine Antibody toMouse IgG2b (antibody to-), fluorecein coupled
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Alternative technique
antibodies, murine, caprine, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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