Goat anti-Mouse IgG (H&L), F(ab)'2 Fragment - Affinity Pure, TRITC Conjugate
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Catalog numberMBS686365
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Price:
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Size5x0.5 mg
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Products_typeSecondary Antibody
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Products_short_name[IgG (H&L), F(ab)'2 Fragment]
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Products_name_syn[Goat anti-Mouse IgG (H&L), F(ab)'2 Fragment - Affinity Pure]
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Other_names[IgG, partial]
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ReactivityMouse. Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin mouse serum immunoglobulins
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SpecificityBased on IEP, this antibody reacts with: heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins
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Purity> 90% based on SDS-PAGE Small amounts of intact IgG may be present. Affinity purified using solid phase Mouse IgG.
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FormLyophilized
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Concentration1.0 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stabilityStore at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_applicationFlow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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TestA high affinity purification column was use to purify Goat anti-Mouse IgG (H L), F(ab)'2 - Pure, TRITC Conjugate by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti-Mouse IgG (H L), F(ab)'2 - Pure, TRITC Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Latin nameCapra aegagrus hircus, Mus musculus
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short nameGoat anti-Mouse IgG (H L), F(ab)'2 - Pure, TRITC Conjugate
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Techniqueaffinity, anti-, Mouse, anti, Goat, Pure, IgG, antibody to, antibodies, goats, IgGs, mouses, pure products
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeIgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. Heavy & Light chains, IgG
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Labeltritc
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SpeciesMouse, Goats, Mouses
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Alternative namecaprine Antibody toMouse Immunoglobulin G (heavy + light chain), F(Antibody)'2 Fragment - protein Pure, TRITC Conjugate
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Alternative techniquemurine, antibodies, purified, caprine, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data