GFAP (Astrocyte & Neural Stem Cell Marker)
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Products_type
Antibody
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Products_short_name
[GFAP]
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Products_name_syn
[Astrocyte or Intermediate Filament Protein; Glial Fibrillary Acidic Protein (GFAP)]
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Other_names
[glial fibrillary acidic protein isoform 2; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein]
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Products_gene_name
[GFAP]
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Other_gene_names
[GFAP; GFAP; ALXDRD; GFAP]
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Clonality
Monoclonal
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Clone
[ASTRO/1974R]
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Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken. Others not known.
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Specificity
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
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Form
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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Storage_stability
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
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Description
For cells, cell lines and tissues in culture till half confluency.
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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Gene target
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Gene symbol
GFAP
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Short name
GFAP (Astrocyte & Neural Stem )
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Technique
Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA.
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Host
Rabbit
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Isotype
IgG
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Alternative name
GFAP (Astrocyte & Neural progenitor cellular detection labelled)
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Tissue
cell, stem
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Gene info
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