FITC-Linked Polyclonal Antibody to Epithelial Cell Adhesion Molecule (EPCAM)
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Catalog number
MBS2053504
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Price
Please ask
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Size
0,1 mg
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Other size
please contact us to order other different size
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the FITC-Linked Polyclonal Antibody Epithelial Adhesion Molecule (EPCAM). For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
EPCAM-DT, EPCAM
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Short name
FITC-Linked Polyclonal Antibody Epithelial Adhesion Molecule (EPCAM)
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Technique
Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Alternative name
fluorecein-Linked polyclonal (antibody to-) to Epithelial cellular Adhesion Molecule (epithelial cellular adhesion molecule)
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Alternative technique
polyclonals, antibodies, fluorescine
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Alternative to gene target
epithelial cell adhesion molecule, DIAR5 and EGP-2 and EGP314 and EGP40 and ESA and HNPCC8 and KS1/4 and KSA and M4S1 and MIC18 and MK-1 and TACSTD1 and TROP1, EPCAM and IDBG-50831 and ENSG00000119888 and 4072, protein complex binding, Cell surfaces, Epcam and IDBG-201437 and ENSMUSG00000045394 and 17075, EPCAM and IDBG-633617 and ENSBTAG00000006474 and 514039
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Tissue
cell
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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