FITC-Linked Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1)

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Properties

If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

Linked ATP Dependent DNA ligase LIG1
Gene symbol

LIG1, VPS9D1, LRIG1, PRKDC, NFATC2, HLA-DOA
Short name

FITC-Linked Polyclonal Antibody ATP Dependent DNA ligase I (LIG1)
Technique

Polyclonal, Antibody, FITC, dna, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Label

FITC
Alternative name

fluorecein-Linked polyclonal (antibody to-) to adenosine triphosphate Dependent Desoxyribonucleic acid ligase I (LIG1)
Alternative technique

polyclonals, antibodies, fluorescine

Identity

HGNC:6598
Gene

LIG1
Long gene name

DNA ligase 1
Synonyms gene name
- ligase I, DNA, ATP-dependent
Synonyms name
- DNA ligase I
Locus

19q13.33
Discovery year

1991-05-09
Entrez gene record

3978
Pubmed identfication
- 1733856
RefSeq identity
- NM_000234
Classification
- Nucleotide excision repair
- DNA ligases
VEGA ID

OTTHUMG00000183187
Locus Specific Databases
- LIG1base: Mutation registry for DNA ligase I deficiency
- LRG_78

Identity

HGNC:17360
Gene

LRIG1
Long gene name

leucine rich repeats and immunoglobulin like domains 1
Synonyms
- LIG-1
- DKFZP586O1624
- LIG1
Synonyms name
- ortholog of mouse integral membrane glycoprotein LIG-1
- leucine-rich repeat protein LRIG1
GenBank acession
- AB050468
Locus

3p14.1
Discovery year

2002-08-23
Entrez gene record

26018
Pubmed identfication
- 11414704
- 12234026
RefSeq identity
- NM_015541
Classification
- I-set domain containing
VEGA ID

OTTHUMG00000158727

Identity

HGNC:7776
Gene

NFATC2
Long gene name

nuclear factor of activated T cells 2
Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
- nuclear factor of activated T-cells 2
Synonyms
- NF-ATP
- NFATp
- NFAT1
GenBank acession
- U43342
- U43341
Locus

20q13.2
Discovery year

1994-11-16
Entrez gene record

4773
Pubmed identfication
- 8202141
RefSeq identity
- NM_012340
Classification
- IPT domain containing
- Nuclear factors of activated T-cells
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000032747

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data