Embryonic Stem Cell Culture Media, Mouse (mES)
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Catalog number
MBS652666
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Price
Please ask
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Size
250 mL
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Other size
please contact us to order other different size
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Description
For cells, cell lines and tissues in culture till half confluency. Differentiation and cell culture media are buffered growth medium solutions or culture medium solutions that can be supplied as a solid or liquid designed to support the growth of microorganisms or cells, or small plants. There are different types of media for growing different types of cells.
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Buiffer
MES buffers are Morpholine Ethane Sulphonic Acid or CAS 4432-31 buffers with compound 2-(N-morpholino)ethane sulfonic acid. Its chemical structure contains a morpholine ring. It has a molecular weight of 195.2 g/mol and the chemical formula is C6H13NO4S. Synonyms include 2-morpholinoethanesulfonic acid; 2-(4-morpholino)ethane sulfonic acid; 2-(N-morpholino)ethane sulfonic acid; 2-(4-morpholino)ethane sulfonic acid; MES; MES hydrate; and morpholine-4-ethanesulfonic acid hydrate. MOPS is a similar pH buffering compound which contains a propane sulfonic moiety instead of an ethane sulfonic one.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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Latin name
Mus musculus
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Gene target
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Short name
Embryonic Stem Culture , Mouse ( )
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Technique
culture, Mouse, mouses
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Embryonic progenitor cellular Culture Media, Mouse (mES)
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Alternative technique
murine
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Tissue
cell, stem
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MeSH Data
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